Michael Reth and his team are studying how the B cell antigen receptor (BCR) and the B cell surface is organized in resting and activated B cells for the sensing of foreign antigens. They learned that the BCR and many B cell surface markers are highly organized at nanoscale distances. In their studies they are using the CRISPR/Cas9 method to rapidly generate loss- or gain-of-function mutants of the Burkitt lymphoma cell line Ramos as specific cell line. They thus have generated Ramos B cells which are lacking all four components of the BCR and replaced the antigen receptor by the spike protein of SARS-CoV-2. This allows them to test for SARS-COV-2 spike specific antibody responses and monoclonal antibodies by flow cytometry. The first results of their assays will be discussed in the presentation. The webinar will be moderated by Rita Carsetti.
https://inmunologia.org.ar/wp-content/uploads/2020/07/webinar2007.jpg 320 1200 Administrador http://inmunologia.org.ar/wp-content/uploads/2016/02/logo-SAI-adaptado.png Administrador2020-07-15 15:04:412020-07-21 16:33:48Webinar: Ramos B cell engineering for the evaluation of the humoral anti-SARS-CoV-2 immunity